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Título Molecular genetics of mammalian cells / edited by Michael M. Gottesman

Publicación San Diego [etc.] : Academic Press, 1987
Ubicación Signatura Tipo de préstamo Estado Notas
 Ciencias.Física/Química-Bioquímica y Biología Molecular y Celular  BQ ME-151    CONSULTAR DEPT  DISPONIBLE
Descripción física XXX, 609 p., [4] p. de lám. : il., fot. ; 24 cm
Colección Methods in enzymology ; 151
Bibliografía Incluye referencias bibliográficas. -- Índices p. 567-609
Contiene: Chinese hamster ovary cells / Michael M Gottesman -- Cultured S49 mouse T lymphoma cells / Theodoor van Daalen Wetters, Philip Coffino -- Liver cell lines / G.J Darlington -- HeLa cell lines / Shin-ichi Akiyama -- Somatic cell genetic analysis of myelomas and hybridomas / Deborah French, Theresa Kelly, Susan Buhl, Matthew D Scharff -- Mouse teratocarcinoma cells / Hedwig Jakob, Jean-François Nicolas -- Amplification of genes in somatic mammalian cells / Robert T Schimke, David S Roos, Peter C Brown -- Replica plating and detection of antigen variants / Susannah Gal -- Drug-resistant mutants: Selection and dominance analysis / Michael M Gottesman -- Suicide selection of mammalian cell mutants / David Patterson, Charles A Waldren -- Use of tritium-labeled precursors to select mutants / Jacques Pouysségur, Arlette Franchi -- Construction of immunotoxins using Pseudomonas exotoxin A / David J.P Fitzgerald -- Isolation of temperature-sensitive mutants / Joseph Hirschberg, Menashe Marcus -- Fluorescence-activated cell sorting of hybrid and transfected cells / Michael E Kamarck -- Isozyme identification of chromosomes in interspecific somatic cell hybrids / Michael J Siciliano, Billie F White -- Principles of electrofusion and electropermeabilization / Ulrich Zimmermann, Howard B Urnovitz -- Cell enucleation, cybrids, reconstituted cells, and nuclear hybrids / Jerry W Shay -- Toxin — antitoxin selection for isolating heterokaryons and cell hybrids / Woodring E Wright -- Chromosome sorting by flow cytometry / Marty Bartholdi, Julie Meyne, Kevin Albright, Mary Luedemann, Evelyn Campbell, Douglas Chritton, Larry L Deaven, L Scott Cram -- Methods for chromosome banding of human and experimental tumors in Vitro / Jeffrey M Trent, Floyd H Thompson -- In situ hybridization of metaphase and prometaphase chromosomes / Susan L Naylor, John R McGill, Bernhard U Zabel -- Gene mapping with sorted chromosomes / Roger V Lebo, Barry D Bruce -- Transfer and selective retention of single specific human chromosomes via microcell-mediated chromosome transfer / Paul J Saxon, Eric J Stanbridge -- Strategies for isolation of mutant genes / Michael M Gottesman -- Use of in-gel DNA renaturation for detection and cloning of amplified genes / Igor B Roninson -- Detection and characterization of specific mRNA by microinjection and complementation of mutant cells / Pin-Fang Lin, David B Brown, Pat Murphy, Masaru Yamaizumi, Frank H Ruddle -- Use of the CAT reporter gene for optimization of gene transfer into eukaryotic cells / C Michael Fordis, Bruce H Howard -- Cosmid vector systems for genomic DNA cloning / Mary McCormick, Max E Gottesman, George A Gaitanaris, Bruce H Howard -- Preparation of a genomic cosmid library / Robert Fleischmann, Mary McCormick, Bruce H Howard -- Colony screening of genomic cosmid libraries / Bruce R Troen -- Rescue of genes transferred from cosmid libraries / Yun-Fai Lau -- Phage-mediated transduction of cDNA expression libraries into mammalian cells / Hiroto Okayama -- Sib selection / Mary McCormick -- Repetitive cloning of mutant genes using locus-specific sticky ends / Lawrence A Chasin, Adelaide M Carothers, Gail Urlaub -- Strategies for mapping and cloning macroregions of mammalian genomes / Cassandra L Smith, Simon K Lawrance, Gerald A Gillespie, Charles R Cantor, Sherman M Weissman, Francis S Collins -- Insertional mutagenesis to isolate genes / Stephen P Goff -- Microcloning of mammalian metaphase chromosomes / Jan-Erik Edström, Rolf Kaiser, Dan Röhme -- Antisense RNA inhibition of endogenous genes / J Timothy Stout, C Thomas Caskey -- In situ detection of transcription in transfected cells using biotin-labeled molecular probes / Gilbert H Smith -- RNA detection and localization in cells and tissue sections by in Situ hybridization of 35S-labeled RNA probes / Mary E Harper, Lisa M Marselle -- Electrophoretic assay for DNA-binding proteins / Alexander Varshavsky
Materia Enzimas -- Análisis
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Mamíferos -- Genética
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Genética Molecular
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Bioquímica -- Técnicas de laboratorio
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Autor secundario Gottesman, Michael M., ed. lit.
En Methods in enzymology.
ISBN 0121820521
ISSN 0076-6879